IL-18 ELISA Kit

Produktnummer ABIN411383

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Ratte IL-18. Es wurde in 7 Publikationen zitiert.

Kurzübersicht für IL-18 ELISA Kit (ABIN411383)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Bindungsspezifität: AA 37-194

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails IL-18 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Rat IL-18 ELISA Kit to quantitate Rat Il18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: H37-S194

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.8%, 4.7%, 4.5%
Inter-Assay Precision (CV%): 7.6%, 5.5%, 6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-18 ELISA Kit (ABIN411383)

Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." in: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

Cao, Fang, Lu, Qian, Ma, He, Pi, Yu, Zhou: "Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats." in: International journal of nanomedicine, Vol. 11, pp. 3331-46, (2017) (PubMed).

Dong, Zhong, Chen, Wu, Zhang, Huang, Yang: "Effect of Acupuncture on Endometrial Angiogenesis and Uterus Dendritic Cells in COH Rats during Peri-Implantation Period." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3647080, (2017) (PubMed).

Tian, Zhu, Yao, Meng, Wang, Xie, Wang: "NLRP3 participates in the regulation of EMT in bleomycin-induced pulmonary fibrosis." in: Experimental cell research, Vol. 357, Issue 2, pp. 328-334, (2017) (PubMed).

Li, Hu, Wang, Zhang, Zhou, Yang, Li, Xiong, Liu, Li, Wu, Zheng: "Autophagy-dependent generation of Axin2+ cancer stem-like cells promotes hepatocarcinogenesis in liver cirrhosis." in: Oncogene, Vol. 36, Issue 48, pp. 6725-6737, (2017) (PubMed).

Meng, Gong, Fang, Song, Wu, Zhou, Qian: "Effect of interferon-γ on NF-κB and cytokine IL-18 and IL-27 in acute pancreatitis." in: Bosnian journal of basic medical sciences, Vol. 13, Issue 2, pp. 114-8, (2014) (PubMed).

Yang, Shen, Chen, Ge: "Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion." in: World journal of gastroenterology, Vol. 11, Issue 29, pp. 4524-9, (2005) (PubMed).

Details zu IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: IL18

Hintergrund:

Background: Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Gene Full Name: interleukin 18

Gen-ID: 29197

UniProt: P97636

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome