INHBA ELISA Kit

Produktnummer ABIN411254

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human INHBA. Es ist 1 Publikation verfügbar.

Kurzübersicht für INHBA ELISA Kit (ABIN411254)

Target: INHBA (Inhibin, beta A (INHBA))

Bindungsspezifität: AA 311-426

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Produktdetails INHBA ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Activin A

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: CHO
Immunogen sequence: G311-S426

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Homologie: Bovine,Monkey,Rabbit

Sensitivität: <12pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: G311-S426

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Tissue Specificity: Uterus, ovary and liver.

Plattentyp: Pre-coated

Protokoll: human Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Activin A amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Activin A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Activin A standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 90, Standard deviation: 4.7, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 281, Standard deviation: 15.7, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 467, Standard deviation: 27.6, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 95, Standard deviation: 7.8, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 293, Standard deviation: 18.5, CV(%): 6.3
• Sample 3: n=24, Mean(pg/ml): 489, Standard deviation: 24.9, CV(%): 5.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für INHBA ELISA Kit (ABIN411254)

Qin, Li, Jiao, Fu, Ye, Ji, Xie, Fan, Xu: "Changes in Blood Components in Aphtha Patients with Excess Heat." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 7219257, (2016) (PubMed).

Details zu INHBA

Target: INHBA (Inhibin, beta A (INHBA))

Andere Bezeichnung: INHBA

Hintergrund:

Protein Function: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Background: Activin A is a homodimer of 14 kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Synonyms: Inhibin, beta A (Activin A, activin AB alpha polypeptide) ,Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ,cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ,INHBA ,hCG_17267 , tcag7.474 ,

Full Gene Name: inhibin, beta A

Cellular Localisation: Secreted.

Gen-ID: 3624

UniProt: A4D1W7

Pathways: Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Autophagie