EGFR ELISA Kit

Produktnummer ABIN3199214

Das Human EGFR ELISA Kit (ABIN3199214) ist ein Colorimetric ELISA Kit zur Detektion von Human EGFR.

Kurzübersicht für EGFR ELISA Kit (ABIN3199214)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.13 pg/mL - 5000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails EGFR ELISA Kit

Untere Nachweisgrenze: 78.13 pg/mL

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human EGFR.

Analytische Methode: Quantitative

Spezifität: EGFR/HER1/ErbB1 ELISA Kit, human

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR/HER1/ErbB1

Hintergrund: The epidermal growth factor receptor (EGFR), also known as ErbB1 and HER1, a type I transmembrane glycoprotein. It exists on the cell surface and is activated by binding of its specific ligands, including EGF and TGF-α.

Gen-ID: 1956

NCBI Accession: NM_005228

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine