VEGFR2/CD309 ELISA Kit
Kurzübersicht für VEGFR2/CD309 ELISA Kit (ABIN3198628)
Target
Alle VEGFR2/CD309 (VEGFR2) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 62.5 pg/mL
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Verwendungszweck
- This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
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Analytische Methode
- Quantitative
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Spezifität
- VEGF R2 / KDR ELISA Kit, Human
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Bestandteile
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- Plate
- Standard
- Diluent
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Benötigtes Material
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Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Probenmenge
- 100 μL
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- protect your eyes
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Lagerung
- 4 °C
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Informationen zur Lagerung
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Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
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- VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))
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Andere Bezeichnung
- VEGF R2 / KDR
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Hintergrund
- Vascular endothelial growth factor receptor-2 (VEGF R2), also known as kinase insert domain receptor (KDR), is a member of the class III subfamily of receptor tyrosine kinases (RTKs). VEGF R2 is more widely distributed and expressed in all vessel-derived endothelial cells in comparison to VEGF R1.
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Gen-ID
- 3791
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NCBI Accession
- NM_002253
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UniProt
- P35968
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Pathways
- RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
Target Alle VEGFR2/CD309 (VEGFR2) ELISA Kits anzeigen
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