NGFB ELISA Kit

Produktnummer ABIN3198504

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human NGFB.

Kurzübersicht für NGFB ELISA Kit (ABIN3198504)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.63 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails NGFB ELISA Kit

Untere Nachweisgrenze: 15.63 pg/mL

Verwendungszweck: This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.

Analytische Methode: Quantitative

Spezifität: β-NGF ELISA Kit, Human

Bestandteile:

• Plate
• Standard
• Diluent

Benötigtes Material: Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
Pipettes and pipette tips.
50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
Multichannel micropipette reservoir.
Beakers, flasks, cylinders necessary for preparation of reagents.
Deionized or distilled water.
Polypropylene test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: protect your eyes

Lagerung: 4 °C

Informationen zur Lagerung: Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit

Details zu NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: beta-NGF

Hintergrund: Nerve growth factor (NGF) is initially in a complex of 3 proteins - α-NGF, β-NGF, and γ-NGF when expressed. This form of NGF is also referred to as proNGF (NGF precursor). The γ subunit of this complex acts as a serine protease, and cleaves the N-terminal of the β subunit, thereby activating the protein into functional NGF.

Gen-ID: 4803

NCBI Accession: NM_002506

UniProt: P01138

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size