Cyclin D1 ELISA Kit

Produktnummer ABIN2947633

Dieses Human Cyclin D1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Cyclin D1 zu quantifizieren.

Kurzübersicht für Cyclin D1 ELISA Kit (ABIN2947633)

Target: Cyclin D1 (CCND1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.16 ng/mL - 10 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails Cyclin D1 ELISA Kit

Untere Nachweisgrenze: 0.16 ng/mL

Verwendungszweck: Human Cyclin D1 (CCND1) ELISA Kit

Analytische Methode: Quantitative

Sensitivität: 0.09 ng/mL

Bestandteile: The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

Benötigtes Material:

• 37 °C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 mL tubes
• Distilled water
• Absorbent filter papers
• 100 mL and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Anwendungsinformationen

Applikationshinweise: Optimal dilutions/concentrations should be determined by the end user.

Kommentare:

The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Plattentyp: Pre-coated

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Cyclin-D1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Cyclin-D1 were tested on 3 different plates, 8 replicates in each plate.

CV (%) = (Standard Deviation / mean) x 100

Intra-Assay: CV<10%

Inter-Assay: CV<10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

Haltbarkeit: 6 months

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling