Kallikrein 12 ELISA Kit

Produktnummer ABIN2859315

Dieses Human Kallikrein 12 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human Kallikrein 12 zu quantifizieren.

Kurzübersicht für Kallikrein 12 ELISA Kit (ABIN2859315)

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Bindungsspezifität: AA 18-248

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156-10.000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails Kallikrein 12 ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human KLK12

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: NSO, A18-N248

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A18-N248

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: human KLK12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been precoated onto 96-well plates. Standards (NSO, A18-N248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK12 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 1409, Standard deviation: 73.26, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 3882, Standard deviation: 213.51, CV(%): 5.5
• Sample 3: n=16, Mean(pg/ml): 6756, Standard deviation: 405.36, CV(%): 6.0,
• Sample 1: n=24, Mean(pg/ml): 1523, Standard deviation: 89.85, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 4129, Standard deviation: 251.86, CV(%): 6.1
• Sample 3: n=24, Mean(pg/ml): 6526, Standard deviation: 443.76, CV(%): 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu Kallikrein 12

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Andere Bezeichnung: KLK12

Hintergrund:

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Synonyms: Kallikrein-12,3.4.21.-,Kallikrein-like protein 5,KLK-L5,KLK12,KLKL5,UNQ669/PRO1303,

Full Gene Name: Kallikrein-12

Cellular Localisation: Secreted.

Gen-ID: 43849

UniProt: Q9UKR0

Pathways: Komplementsystem