Kallikrein 12 ELISA Kit

Produktnummer ABIN6719901

Produktdetails Kallikrein 12 ELISA Kit

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: DKFZp686H1078, Kallikrein like protein 5, Kallikrein related peptidase 12, Kallikrein12, KLK 12, KLK L5, KLK12, KLKL5, MGC42603

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Gene Name: KLK12

Production: Natural and recombinant human KLK12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A18-N248

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Kallikrein 12

Target: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Andere Bezeichnung: KLK12 (KLK12 Produkte)

Synonyme: KLK12 ELISA Kit, 2310008B01Rik ELISA Kit, AI324852 ELISA Kit, KLK-L5 ELISA Kit, KLKL5 ELISA Kit, kallikrein-12 ELISA Kit, kallikrein related-peptidase 12 ELISA Kit, kallikrein related peptidase 12 ELISA Kit, LOC611766 ELISA Kit, Klk12 ELISA Kit, KLK12 ELISA Kit

Gen-ID: 43849

NCBI Accession: NP_062544

UniProt: Q9UKR0

Pathways: Komplementsystem