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ADAM8 ELISA Kit

Human ADAM8 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human ADAM8.
Produktnummer ABIN2859283

Kurzübersicht für ADAM8 ELISA Kit (ABIN2859283)

Target

Alle ADAM8 ELISA Kits anzeigen
ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

Bindungsspezifität

AA 1-497

Reaktivität

  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
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Human

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Detektionsbereich

62.5-4000 pg/mL

Applikation

ELISA

Proben

Cell Culture Supernatant, Serum
  • Untere Nachweisgrenze

    62.5 pg/mL

    Verwendungszweck

    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAM8

    Marke

    PicoKine™

    Analytische Methode

    Quantitative

    Spezifität

    NSO, M1-P497

    Kreuzreaktivität (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität

    <10pg/mL

    Benötigtes Material

    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

    Immunogen

    Expression system for standard: NSO
    Immunogen sequence: M1-P497
  • Applikationshinweise

    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

    Kommentare

    Tissue Specificity: Expressed on neutrophils and monocytes.

    Plattentyp

    Pre-coated

    Protokoll

    human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.

    Testdurchführung

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

    Testpräzision

    • Sample 1: n=16, Mean(ng/ml): 0.22, Standard deviation: 0.013, CV(%): 5.7
    • Sample 2: n=16, Mean(ng/ml): 1.47, Standard deviation: 0.090, CV(%): 6.1
    • Sample 3: n=16, Mean(ng/ml): 2.34, Standard deviation: 0.185, CV(%): 7.9,
    • Sample 1: n=24, Mean(ng/ml): 0.27, Standard deviation: 0.018, CV(%): 6.8
    • Sample 2: n=24, Mean(ng/ml): 1.56, Standard deviation: 0.115, CV(%): 7.4
    • Sample 3: n=24, Mean(ng/ml): 2.58, Standard deviation: 0.214, CV(%): 8.3

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Handhabung

    Avoid multiple freeze-thaw cycles.

    Lagerung

    -20 °C,4 °C

    Informationen zur Lagerung

    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

    Haltbarkeit

    12 months
  • Target Alle ADAM8 ELISA Kits anzeigen

    ADAM8 (ADAM Metallopeptidase Domain 8 (ADAM8))

    Andere Bezeichnung

    ADAM8

    Hintergrund

    Protein Function: Possible involvement in extravasation of leukocytes.

    Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

    Synonyms: Disintegrin and metalloproteinase domain-containing protein 8,ADAM 8,3.4.24.-,Cell surface antigen MS2,CD156a,ADAM8,MS2,

    Full Gene Name: Disintegrin and metalloproteinase domain-containing protein 8

    Cellular Localisation: Membrane, Single-pass type I membrane protein.

    Gen-ID

    101

    UniProt

    P78325

    Pathways

    Activation of Innate immune Response, M Phase
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