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GDNF ELISA Kit

GDNF Reaktivität: Ratte Colorimetric Sandwich ELISA 60-15.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2748140
  • Target Alle GDNF ELISA Kits anzeigen
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Reaktivität
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    60-15.000 pg/mL
    Untere Nachweisgrenze
    60 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Rat GDNF ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair detects rat and human GDNF. Other species not determined.
    Sensitivität
    60 pg/mL
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Testdurchführung

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle GDNF ELISA Kits anzeigen
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Andere Bezeichnung
    GDNF (GDNF Produkte)
    Synonyme
    gdnf ELISA Kit, GDNF ELISA Kit, AI385739 ELISA Kit, gdnfa ELISA Kit, hfb1-gdnf ELISA Kit, ATF1 ELISA Kit, ATF2 ELISA Kit, HFB1-GDNF ELISA Kit, HSCR3 ELISA Kit, glial cell derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor a ELISA Kit, glial cell line derived neurotrophic factor ELISA Kit, glial cell derived neurotrophic factor L homeolog ELISA Kit, Gdnf ELISA Kit, gdnfa ELISA Kit, GDNF ELISA Kit, gdnf.L ELISA Kit
    Hintergrund
    Glial cell line-derived neurotrophic factor (Astrocyte-derived trophic factor) (ATF)
    Gen-ID
    25453
    UniProt
    Q07731
    Pathways
    RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration
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