EGF ELISA Kit

Produktnummer ABIN2708197

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human EGF.

Kurzübersicht für EGF ELISA Kit (ABIN2708197)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9 pg/mL - 250 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma, Urine, Tissue Homogenate

Produktdetails EGF ELISA Kit

Untere Nachweisgrenze: 3.9 pg/mL

Verwendungszweck: Sandwich ELISA for quantitative detection of Human EGF concentrations.

Analytische Methode: Quantitative

Sensitivität: 2 pg/mL

Bestandteile:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped and store at 4°C for 6 months, store at -20°C for one year.

Haltbarkeit: 6-12 months

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: Alternative Names: Pro-epidermal growth factor, EGF

Gen-ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation