FLT4 ELISA Kit

Produktnummer ABIN2703571

Dieses Maus FLT4 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus FLT4 zu quantifizieren.

Kurzübersicht für FLT4 ELISA Kit (ABIN2703571)

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.2-50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails FLT4 ELISA Kit

Untere Nachweisgrenze: 0.2 ng/mL

Verwendungszweck: Mouse VEGFR3 ELISA Kit for Cell Culture Supernatants, EDTA and/or Citrate treated plasma, and Serum samples. Heparin is not recommended.

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Mouse Vascular endothelial growth factor receptor 3.

Sensitivität: 0.2 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FLT4

Target: FLT4 (Fms-Related Tyrosine Kinase 4 (FLT4))

Andere Bezeichnung: VEGFR3

Hintergrund: Gene Names: Flt4 Flt-4 Vegfr3
Protein names: Vascular endothelial growth factor receptor 3 (VEGFR-3) (EC 2.7.10.1) (Fms-like tyrosine kinase 4) (FLT-4) (Tyrosine-protein kinase receptor FLT4)

Gen-ID: 14257

UniProt: P35917

Pathways: RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling