MAPT ELISA Kit

Produktnummer ABIN2703476

Human MAPT ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MAPT.

Kurzübersicht für MAPT ELISA Kit (ABIN2703476)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 100 pg/mL - 25000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails MAPT ELISA Kit

Untere Nachweisgrenze: 100 pg/mL

Verwendungszweck: Human MAPT (Tau) ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human Tau. Other species not determined.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Suggested Plasma Dilution: 2 fold

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

Collect plasma using EDTA and/or Citrate as the anticoagulant. Heparin is not recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Andere Bezeichnung: tau

Hintergrund:

Gene Symbols: MAPT, MAPTL, MTBT1, TAU

Protein Name (Synonyms): Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

Gen-ID: 4137

UniProt: P10636

Pathways: MAPK Signalweg, Microtubule Dynamics, M Phase, Regulation of Cell Size