PGLYRP1 ELISA Kit

Produktnummer ABIN2703415

Produktdetails PGLYRP1 ELISA Kit

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 6-1.500 pg/mL

Untere Nachweisgrenze: 6 pg/mL

Applikation: ELISA

Verwendungszweck: Human PGRPs ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human PGRPs, less than 0.05 % cross-reactivity with human PGRP-1 beta and mouse PGRPs is observed.

Sensitivität: 6 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples100 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PGLYRP1

Target: PGLYRP1 (Peptidoglycan Recognition Protein 1 (PGLYRP1))

Andere Bezeichnung: PGRPs (PGLYRP1 Produkte)

Synonyme: PGLYRP1 ELISA Kit, PGLYRP ELISA Kit, pgrp ELISA Kit, tag7 ELISA Kit, pgrps ELISA Kit, pglyrp ELISA Kit, pgrp-s ELISA Kit, tnfsf3l ELISA Kit, MGC108330 ELISA Kit, MGC108469 ELISA Kit, PGRP-SC1a ELISA Kit, si:dkey-113g17.4 ELISA Kit, si:ch211-202c21.3 ELISA Kit, PGRP ELISA Kit, PGRP-S ELISA Kit, PGRPS ELISA Kit, TAG7 ELISA Kit, TNFSF3L ELISA Kit, Pglyrp ELISA Kit, Tag7 ELISA Kit, Tasg7 ELISA Kit, Tnfsf3l ELISA Kit, Pgrp ELISA Kit, peptidoglycan recognition protein 1 ELISA Kit, peptidoglycan recognition protein 1 L homeolog ELISA Kit, peptidoglycan recognition protein 5 ELISA Kit, PGLYRP1 ELISA Kit, pglyrp1.L ELISA Kit, pglyrp1 ELISA Kit, pglyrp5 ELISA Kit, Pglyrp1 ELISA Kit

Hintergrund: Gene Names: PGLYRP1 PGLYRP PGRP TNFSF3L SBBI68 UNQ639/PRO1269
Protein names: Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S)

Gen-ID: 8993

UniProt: O75594

Pathways: Activation of Innate immune Response