APOA1 ELISA Kit

Produktnummer ABIN2702827

Dieses Human und Rhesusaffen APOA1 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human und Rhesusaffen APOA1 in Cell Culture Supernatant, Plasma und Serum zu quantifizieren.

Kurzübersicht für APOA1 ELISA Kit (ABIN2702827)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Human, Rhesusaffen

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.08-20 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum

Produktdetails APOA1 ELISA Kit

Untere Nachweisgrenze: 0.08 ng/mL

Verwendungszweck: Human ApoA ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects human ApoA1. Cross-reacts with ApoA1 from rhesus and cynomolgus macaques. Other species not determined.

Sensitivität: 0.08 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples1,000,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

For example, add 1 μL of serum or plasma into a tube with 999 μL 1X Assay Diluent to prepare a 1,000-fold diluted sample. Mix thoroughly and then pipette 1 μL of prepared 1,000-fold diluted sample into a tube with 999 μL 1X Assay Diluent to prepare a final 1,000,000-fold diluted sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: APOA1

Hintergrund: Gene Names: APOA1
Protein names: Apolipoprotein A-I (Apo-AI) (ApoA-I) (Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I (ProapoA-I), Truncated apolipoprotein A-I (Apolipoprotein A-I(1-242))]

Gen-ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism