IL-8 ELISA Kit

Produktnummer ABIN2648705

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL-8.

Kurzübersicht für IL-8 ELISA Kit (ABIN2648705)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails IL-8 ELISA Kit

Verwendungszweck: Human Interleukin 8 (IL-8) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 8 (IL-8) concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Sensitivität: 4 pg/ml

Produktmerkmale: Human Interleukin 8 (IL-8) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody-IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Andere Bezeichnung: IL-8

Pathways: TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagie