EGF ELISA Kit

Produktnummer ABIN2648643

Dieses Human EGF ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human EGF zu quantifizieren.

Kurzübersicht für EGF ELISA Kit (ABIN2648643)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Produktdetails EGF ELISA Kit

Verwendungszweck: Human Epidermal Growth Factor (EGF) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Epidermal Growth Factor (EGF) concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Produktmerkmale: Human Epidermal Growth Factor (EGF) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 3.5 h

Plattentyp: Pre-coated

Protokoll: Human Epidermal Growth Factor (EGF) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for EGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGF present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for EGF is added to the wells and binds to the combination of capture antibody- EGF in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of EGF present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven EGF standard dilutions and EGF sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation