IL4 Receptor ELISA Kit

Produktnummer ABIN2648537

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human IL4 Receptor.

Kurzübersicht für IL4 Receptor ELISA Kit (ABIN2648537)

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Reaktivität: Human

Nachweismethode: Colorimetric

Applikation: ELISA

Produktdetails IL4 Receptor ELISA Kit

Verwendungszweck: Human IL-4R ELISA is to be used for the in-vitro quantitative determination of soluble IL-4 receptor molecule (CD124) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human IL-4R.

Analytische Methode: Quantitative

Produktmerkmale: Human IL-4R ELISA Assay kit is for Research Use Only.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: The IL-4R Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations and unknowns are pipetted into these wells. During the first incubation, the IL-4R antigen and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme ( streptavidin-peroxydase ) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-4R present in the samples.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu IL4 Receptor

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: CD124 / IL-4R

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process