VEGFR2/CD309 ELISA Kit

Produktnummer ABIN2044781

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Schwein VEGFR2/CD309.

Kurzübersicht für VEGFR2/CD309 ELISA Kit (ABIN2044781)

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Produktdetails VEGFR2/CD309 ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Sensitivität: < 0.62 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor Receptor 2 (VEGFR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu VEGFR2/CD309

Target: VEGFR2/CD309 (VEGFR2) (VEGF Receptor 2 (VEGFR2))

Andere Bezeichnung: Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling