SUMO1 ELISA Kit

Produktnummer ABIN2044567

Human SUMO1 ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human SUMO1.

Kurzübersicht für SUMO1 ELISA Kit (ABIN2044567)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails SUMO1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.061 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Small Ubiquitin Related Modifier Protein 1 (SUMO1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Small Ubiquitin Related Modifier Protein 1 (SUMO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Ubiquitin Related Modifier Protein 1 (SUMO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Small Ubiquitin Related Modifier Protein 1 (SUMO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway