RAC1 ELISA Kit

Produktnummer ABIN2044385

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human RAC1.

Kurzübersicht für RAC1 ELISA Kit (ABIN2044385)

Target: RAC1

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails RAC1 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.060 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Related C3 Botulinum Toxin Substrate 1 (Rac1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu RAC1

Target: RAC1

Andere Bezeichnung: Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)

Pathways: WNT Signalweg, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling