MAPK12 ELISA Kit

Produktnummer ABIN2043983

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Human MAPK12.

Kurzübersicht für MAPK12 ELISA Kit (ABIN2043983)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Applikation: ELISA

Produktdetails MAPK12 ELISA Kit

Untere Nachweisgrenze: 0.15 ng/mL

Sensitivität: < 0.072 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Mitogen Activated Protein Kinase 12 (MAPK12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 12 (MAPK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mitogen Activated Protein Kinase 12 (MAPK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Andere Bezeichnung: Mitogen Activated Protein Kinase 12 (MAPK12)

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteine