CD11b ELISA Kit

Produktnummer ABIN2043771

Dieses Human CD11b ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CD11b zu quantifizieren.

Kurzübersicht für CD11b ELISA Kit (ABIN2043771)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Applikation: ELISA

Produktdetails CD11b ELISA Kit

Untere Nachweisgrenze: 0.31 ng/mL

Sensitivität: < 0.125 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Integrin Alpha M (ITGaM) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha M (ITGaM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha M (ITGaM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha M (ITGaM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Integrin Alpha M (ITGaM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation