IL-2 ELISA Kit

Produktnummer ABIN2043675

Das Human IL-2 ELISA Kit (ABIN2043675) ist ein Colorimetric ELISA Kit zur Detektion von Human IL-2.

Kurzübersicht für IL-2 ELISA Kit (ABIN2043675)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78-50 pg/mL

Applikation: ELISA

Produktdetails IL-2 ELISA Kit

Untere Nachweisgrenze: 0.78 pg/mL

Sensitivität: < 0.28 pg/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: High Sensitivity Interleukin 2 (IL2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 (IL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 (IL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 (IL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 2 (IL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation