APOA1 ELISA Kit

Produktnummer ABIN2042917

Hund APOA1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Hund APOA1.

Kurzübersicht für APOA1 ELISA Kit (ABIN2042917)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reaktivität: Hund

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25-2000 ng/mL

Applikation: ELISA

Produktdetails APOA1 ELISA Kit

Untere Nachweisgrenze: 31.25 ng/mL

Sensitivität: < 14.8 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Apolipoprotein A1 (APOA1) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Andere Bezeichnung: Apolipoprotein A1 (APOA1)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism