TGFA ELISA Kit

Produktnummer ABIN2042674

Human TGFA ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human TGFA.

Kurzübersicht für TGFA ELISA Kit (ABIN2042674)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails TGFA ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is cross-reactivates with TGF 2, TGF 3, TGF 5 <1 % .

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-TGF alpha, Human
Lyophilized recombinant TGF alpha, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Human TGF alpha in cell culture supernates, serum, plasma (heparin, EDTA) and human milk.Transforming growth factor alpha (TGF- ) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGF stimulates neural cell proliferation in the adult injured brain.1 Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma.2 Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha

Gen-ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway