NGFB ELISA Kit

Produktnummer ABIN2042621

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Ratte NGFB.

Kurzübersicht für NGFB ELISA Kit (ABIN2042621)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Applikation: ELISA

Produktdetails NGFB ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-NGF/NGF beta, Rat
Lyophilized recombinant NGF/NGF beta, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Rat NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Gen-ID: 310738

UniProt: P25427

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size