NGFB ELISA Kit

Produktnummer ABIN2042620

Maus NGFB ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus NGFB.

Kurzübersicht für NGFB ELISA Kit (ABIN2042620)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2-2000 pg/mL

Applikation: ELISA

Produktdetails NGFB ELISA Kit

Untere Nachweisgrenze: 31.2 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-NGF/NGF beta, Mouse
Lyophilized recombinant NGF/NGF beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Gen-ID: 18049

UniProt: P01139

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size