MME ELISA Kit

Produktnummer ABIN2042615

Dieses Colorimetric ELISA-Kit wurde entwickelt für die quantitative Messung von Maus MME.

Kurzübersicht für MME ELISA Kit (ABIN2042615)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 93.7-6000 pg/mL

Applikation: ELISA

Produktdetails MME ELISA Kit

Untere Nachweisgrenze: 93.7 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile: 96-well plate pre-coated with anti-Neprilysin/CD10, Mouse
Lyophilized recombinant Neprilysin/CD10, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse Neprilysin/CD10 in cell culture supernates, serum and plasma (heparin, EDTA).Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), CD10, and common acute lymphoblastic leukemia antigen (CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu MME

Target: MME (Membrane Metallo-Endopeptidase (MME))

Andere Bezeichnung: Neprilysin/CD10

Substanzklasse: Chemical

Gen-ID: 17380

UniProt: Q61391

Pathways: RTK Signalweg, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration