IL-3 ELISA Kit

Produktnummer ABIN2042547

Dieses Maus IL-3 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Maus IL-3 zu quantifizieren.

Kurzübersicht für IL-3 ELISA Kit (ABIN2042547)

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Produktdetails IL-3 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-IL-3, Mouse
Lyophilized recombinant IL-3, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Mouse IL-3 in cell culture supernates and serum.The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15kD.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL-3

Gen-ID: 16187

UniProt: P01586

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie