EGF ELISA Kit

Produktnummer ABIN2042445

Dieses Ratte EGF ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Ratte EGF zu quantifizieren.

Kurzübersicht für EGF ELISA Kit (ABIN2042445)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Produktdetails EGF ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile: 96-well plate pre-coated with anti-EGF, Rat
Lyophilized recombinant EGF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Sandwich ELISA quantitative detection kit for Rat EGF in cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine.Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Gen-ID: 25313

UniProt: P07522

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation