LBP ELISA Kit

Produktnummer ABIN1889416

Maus LBP ELISA-Kit Colorimetric-Assay zur Quantifizierung von Maus LBP und wurde in 1 Publikation erwähnt.

Kurzübersicht für LBP ELISA Kit (ABIN1889416)

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Bindungsspezifität: AA 25-481

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56-100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Produktdetails LBP ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse LBP

Marke: PicoKine™

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: G25-V481

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <50pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: G25-V481

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse LBP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for LBP has been precoated onto 96-well plates. Standards(NSO, G25-V481) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LBP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse LBP amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse LBP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse LBP standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(ng/ml): 5.61, Standard deviation: 0.32, CV(%): 5.7
• Sample 2: n=16, Mean(ng/ml): 33.8, Standard deviation: 1.55, CV(%): 4.6
• Sample 3: n=16, Mean(ng/ml): 68.2, Standard deviation: 3.41, CV(%): 5,
• Sample 1: n=24, Mean(ng/ml): 5.32, Standard deviation: 0.46, CV(%): 8.6
• Sample 2: n=24, Mean(ng/ml): 36.5, Standard deviation: 1.93, CV(%): 5.3
• Sample 3: n=24, Mean(ng/ml): 74.9, Standard deviation: 4.81, CV(%): 6.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für LBP ELISA Kit (ABIN1889416)

Wang, Shen, Zhang, Wang, Xu, Chen, Chen, Yang, He, Wang, Su, Cheng, Zhao, Wang: "Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5." in: Scientific reports, Vol. 6, pp. 22875, (2017) (PubMed).

Details zu LBP

Target: LBP (Lipopolysaccharide Binding Protein (LBP))

Andere Bezeichnung: LBP

Hintergrund:

Protein Function: Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (By similarity). .

Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Synonyms: Lipopolysaccharide-binding protein,LBP,Lbp,

Full Gene Name: Lipopolysaccharide-binding protein

Cellular Localisation: Secreted.

Gen-ID: 16803

UniProt: Q61805

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process