CD40 Ligand ELISA Kit (CD40LG)

Details for Product CD40LG ELISA Kit No. ABIN1889305
Antigen
Epitop
AA 61-260
Reaktivität
Maus
Alternativen
Kits mit alternativen Reaktivitäten:
6
6
6
5
5
5
3
3
3
2
1
1
1
1
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
62.5-4000 pg/mL
Untere Nachweisgrenze
62.5 pg/mL
Applikation
ELISA
Optionen
Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD40L
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: E61-L260
Spezifität Expression system for standard: NSO
Immunogen sequence: E61-L260
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Antigen
Andere Bezeichnung CD40LG (CD40LG ELISA Kit Abstract)
Hintergrund

Protein Function: Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity). .

Background: CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Synonyms: CD40 ligand,CD40-L,T-cell antigen Gp39,TNF-related activation protein,TRAP,Tumor necrosis factor ligand superfamily member 5,CD154,CD40 ligand, membrane form,CD40 ligand, soluble form,Cd40lg,Cd40l, Tnfsf5,

Full Gene Name: CD40 ligand

Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
Gen-ID 21947
UniProt P27548
Pathways NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
Applikations-hinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Tissue Specificity: Specifically expressed on activated CD4+ T- lymphocytes.

Plattentyp Pre-coated
Protokoll mouse CD40L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD40L has been precoated onto 96-well plates. Standards(NSO, E61-L260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD40L amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD40L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD40L standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 537, Standard deviation: 28.5, CV(%): 5.3
  • Sample 2: n=16, Mean(pg/ml): 1562, Standard deviation: 71.9, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 3025, Standard deviation: 172.4, CV(%): 5.7,
  • Sample 1: n=24, Mean(pg/ml): 562, Standard deviation: 36.53, CV(%): 6.5
  • Sample 2: n=24, Mean(pg/ml): 1675, Standard deviation: 97.15, CV(%): 5.8
  • Sample 3: n=24, Mean(pg/ml): 3140, Standard deviation: 207.24, CV(%): 6.6
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for CD40 Ligand (CD40LG) ELISA Kit (ABIN1889305) Mouse CD40L PicoKine ELISA Kit standard curve