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Prolactin ELISA Kit

PRL Reaktivität: Maus AA 32-228 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (citrate), Plasma (heparin), Serum
Produktnummer ABIN1672761
  • Target Alle Prolactin (PRL) ELISA Kits anzeigen
    Prolactin (PRL)
    Bindungsspezifität
    AA 32-228
    Reaktivität
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156-10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Verwendungszweck
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Prolactin
    Marke
    PicoKine™
    Proben
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)
    Analytische Methode
    Quantitative
    Spezifität
    Expression system for standard: E.coli
    Immunogen sequence: L32-C228
    Kreuzreaktivität (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivität
    <10pg/mL
    Benötigtes Material
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: E.coli
    Immunogen sequence: L32-C228
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  • Applikationshinweise
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plattentyp
    Pre-coated
    Protokoll
    mouse Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Prolactin has been precoated onto 96-well plates. Standards(E.coli, L32-C228) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Prolactin amount of sample captured in plate.
    Testdurchführung

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Prolactin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Prolactin standard solution and each sample be measured in duplicate.

    Testpräzision
    • Sample 1: n=16, Mean(pg/ml): 1490, Standard deviation: 67.05, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 2929, Standard deviation: 158.2, CV(%): 5.4
    • Sample 3: n=16, Mean(pg/ml): 4543, Standard deviation: 177.2, CV(%): 3.9,
    • Sample 1: n=24, Mean(pg/ml): 1272, Standard deviation: 99.22, CV(%): 7.8
    • Sample 2: n=24, Mean(pg/ml): 2683, Standard deviation: 153, CV(%): 5.7
    • Sample 3: n=24, Mean(pg/ml): 4726, Standard deviation: 246, CV(%): 5.2
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Avoid multiple freeze-thaw cycles.
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Haltbarkeit
    12 months
  • Xia, Qi, Zou, Yao: "Influenza A(H1N1) vaccination during early pregnancy transiently promotes hippocampal neurogenesis and working memory. Involvement of Th1/Th2 balance." in: Brain research, Vol. 1592, pp. 34-43, (2015) (PubMed).

  • Target Alle Prolactin (PRL) ELISA Kits anzeigen
    Prolactin (PRL)
    Andere Bezeichnung
    PRL (PRL Produkte)
    Synonyme
    PRL ELISA Kit, prolactin ELISA Kit, PRLB ELISA Kit, PRLSD1 ELISA Kit, Prl1a1 ELISA Kit, Prol ELISA Kit, RATPRLSD1 ELISA Kit, RNPROL ELISA Kit, AV290867 ELISA Kit, prl ELISA Kit, prolactin ELISA Kit, prolactin, gene 2 L homeolog ELISA Kit, PROLACTIN protein ELISA Kit, prolactin-like ELISA Kit, PRL ELISA Kit, prl.2.L ELISA Kit, Prl ELISA Kit, LOC100136792 ELISA Kit, LOC100136580 ELISA Kit, PROLACTIN ELISA Kit, LOC101843376 ELISA Kit
    Hintergrund

    Protein Function: Prolactin acts primarily on the mammary gland by promoting lactation.

    Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

    Synonyms: Prolactin,PRL,Prl,

    Full Gene Name: Prolactin

    Cellular Localisation: Secreted.
    Gen-ID
    19109
    UniProt
    P06879
    Pathways
    JAK-STAT Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
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