Prolactin ELISA Kit

Produktnummer ABIN1672760

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Prolactin. Es wurde in 3 Publikationen zitiert.

Kurzübersicht für Prolactin ELISA Kit (ABIN1672760)

Target: Prolactin (PRL)

Bindungsspezifität: AA 29-227

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate)

Produktdetails Prolactin ELISA Kit

Untere Nachweisgrenze: 156 pg/mL

Verwendungszweck: Sandwich Human Prolactin ELISA Kit to quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: L29-C227

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.5%, 4.8%, 7.2%
Inter-Assay Precision (CV%): 7.9%, 6.7%, 7.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für Prolactin ELISA Kit (ABIN1672760)

Garrido-Gomez, Dominguez, Quiñonero, Diaz-Gimeno, Kapidzic, Gormley, Ona, Padilla-Iserte, McMaster, Genbacev, Perales, Fisher, Simón: "Defective decidualization during and after severe preeclampsia reveals a possible maternal contribution to the etiology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 40, pp. E8468-E8477, (2018) (PubMed).

Wu, Kimura, Zheng, Ishida, Niwa, Hirata, Takebayashi, Takashima, Takahashi, Kushima, Zhang, Murakami: "Chronic endometritis modifies decidualization in human endometrial stromal cells." in: Reproductive biology and endocrinology : RB&E, Vol. 15, Issue 1, pp. 16, (2017) (PubMed).

Xia, Qi, Zou, Yao: "Influenza A(H1N1) vaccination during early pregnancy transiently promotes hippocampal neurogenesis and working memory. Involvement of Th1/Th2 balance." in: Brain research, Vol. 1592, pp. 34-43, (2015) (PubMed).

Details zu Prolactin

Target: Prolactin (PRL)

Andere Bezeichnung: PRL

Hintergrund:

Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.

Gene Full Name: prolactin

Gen-ID: 5617

UniProt: P01236

Pathways: JAK-STAT Signalweg, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus