GST Antikörper

Produktnummer ABIN99862

Hersteller Produkt- Nr.: 600-101-200

Der Ziege Polyklonal Anti-GST-Antikörper wurde für WB, ELISA, IP und IHC validiert. Er ist geeignet, GST in Proben von Schistosoma japonicum zu detektieren. Es sind 7+ Publikationen verfügbar.

Kurzübersicht für GST Antikörper (ABIN99862)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Produktdetails anti-GST Antikörper

Hersteller Produkt- Nr.: 600-101-200

Hersteller: Rockland

Verwendungszweck: GST Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

Produktmerkmale: Synonyms: goat anti-GST antibody, Glutathione-S-Transferase

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:10,000

Application Note: Anti-GST has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This antibody has also been reported to be suitable for immobilization in Label-free Interaction Analysis (Biocore).

Western Blot Dilution: 1:1,000 - 1:10,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store GST antibody at 4° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-GST Antikörper (ABIN99862)

Licht, Hartl, Amman, Anrather, Janisiw, Jantsch: "Inosine induces context-dependent recoding and translational stalling." in: Nucleic acids research, Vol. 47, Issue 1, pp. 3-14, (2019) (PubMed).

Machida, Kubota, Kobayashi, Iwadate, Saeki, Yamaura, Nomura, Takiguchi, Hiwasa: "Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX)." in: Journal of translational medicine, Vol. 13, pp. 71, (2016) (PubMed).

Hang, Lin, Grant, Fleurkens, Villiger, Soos, Morbidelli, Woods, Gauss, Aebi: "Analysis of site-specific N-glycan remodeling in the endoplasmic reticulum and the Golgi." in: Glycobiology, Vol. 25, Issue 12, pp. 1335-49, (2016) (PubMed).

Pitoniak, Chavel, Chow, Smith, Camara, Karunanithi, Li, Wolfe, Cullen: "Cdc42p-interacting protein Bem4p regulates the filamentous-growth mitogen-activated protein kinase pathway." in: Molecular and cellular biology, Vol. 35, Issue 2, pp. 417-36, (2015) (PubMed).

Karunanithi, Cullen: "The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae." in: Genetics, Vol. 192, Issue 3, pp. 869-87, (2013) (PubMed).

Rizzolio, Lucchetti, Caligiuri, Marchesi, Caputo, Klein-Szanto, Bagella, Castronovo, Giordano: "Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1." in: Cell death and differentiation, Vol. 19, Issue 7, pp. 1152-61, (2012) (PubMed).

Sagermann, Chapleau, DeLorimier, Lei: "Using affinity chromatography to engineer and characterize pH-dependent protein switches." in: Protein science : a publication of the Protein Society, Vol. 18, Issue 1, pp. 217-28, (2009) (PubMed).

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST

Hintergrund: Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.

UniProt: P08515