GST Antikörper
Kurzübersicht für GST Antikörper (ABIN966238)
Target
Alle GST Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Spezifität
- Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
-
Aufreinigung
- Antibodies are purified by protein A affinity chromatography
-
Isotyp
- IgG1
-
-
-
-
Applikationshinweise
-
Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Lagerung
- -20 °C
-
-
-
: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, (1988) (PubMed).
-
-
- GST (Glutathione S Transferase (GST))
-
Andere Bezeichnung
- Glutathione-S-Transferase (GST)
-
Hintergrund
- Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Target
-