Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

GST Antikörper

Dieses Anti-GST-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von GST in WB, ELISA und IHC. Geeignet für Schistosoma japonicum. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Rockland
Produktnummer ABIN99861
Hersteller Produkt- Nr.: 100-101-200

Kurzübersicht für GST Antikörper (ABIN99861)

Target

Alle GST Antikörper anzeigen
GST (Glutathione S Transferase (GST))

Reaktivität

  • 96
  • 29
  • 14
  • 8
  • 6
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Schistosoma japonicum

Wirt

  • 65
  • 53
  • 48
  • 2
  • 1
  • 1
Ziege

Klonalität

  • 99
  • 69
Polyklonal

Konjugat

  • 73
  • 11
  • 11
  • 10
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GST Antikörper ist unkonjugiert

Applikation

  • 145
  • 75
  • 38
  • 32
  • 26
  • 25
  • 16
  • 15
  • 10
  • 9
  • 8
  • 6
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Hersteller Produkt- Nr.

    100-101-200

    Hersteller

    Rockland

    Verwendungszweck

    GST Antibody

    Kreuzreaktivität (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].

    Produktmerkmale

    Synonyms: goat anti-GST Antibody, GST, Glutathione-S-Transferase

    Aufreinigung

    This product was prepared from monospecific antiserum by a delipidation and defibrination.

    Immunogen

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:8,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    90 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Haltbarkeit

    12 months
  • Miller, Tejada, Gazzano-Santoro: "Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).

  • Target

    GST (Glutathione S Transferase (GST))

    Andere Bezeichnung

    GST

    Hintergrund

    Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
Sie sind hier:
Chat with us!