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GST Antikörper

GST Reaktivität: Schistosoma japonicum WB, ELISA, IHC Wirt: Ziege Polyclonal unconjugated
Rockland
Produktnummer ABIN99861
Hersteller Produkt- Nr.: 100-101-200
  • Target Alle GST Antikörper anzeigen
    GST (Glutathione S Transferase (GST))
    Reaktivität
    • 96
    • 29
    • 14
    • 9
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Schistosoma japonicum
    Wirt
    • 61
    • 54
    • 46
    • 2
    • 1
    • 1
    • 1
    Ziege
    Klonalität
    • 99
    • 65
    Polyklonal
    Konjugat
    • 74
    • 11
    • 11
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GST Antikörper ist unkonjugiert
    Applikation
    • 147
    • 77
    • 37
    • 30
    • 26
    • 25
    • 15
    • 11
    • 10
    • 9
    • 8
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Hersteller Produkt- Nr.
    100-101-200
    Hersteller
    Rockland
    Verwendungszweck
    GST Antibody
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    Produktmerkmale
    Synonyms: goat anti-GST Antibody, GST, Glutathione-S-Transferase
    Aufreinigung
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    Immunogen

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

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    Discover our top product GST Primärantikörper
  • Applikationshinweise

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:8,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    90 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Miller, Tejada, Gazzano-Santoro: "Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).

  • Target
    GST (Glutathione S Transferase (GST))
    Andere Bezeichnung
    GST (GST Produkte)
    Hintergrund
    Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
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