CREB1 Antikörper (N-Term)

Produktnummer ABIN97864

Hersteller Produkt- Nr.: 100-401-195

Produktdetails anti-CREB1 Antikörper

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CREB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Gel Shift (GS)

Hersteller Produkt- Nr.: 100-401-195

Hersteller: Rockland

Verwendungszweck: CREB-1 Antibody

Produktmerkmale: Synonyms: rabbit anti-CREB1 Antibody, rabbit anti-p43 antibody, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, p43

Aufreinigung: Anti-CREB-1 was prepared from monospecific antiserum by delipidation and immunoadsorption against an E.coli lysate immobilized on agarose beads.

Sterilität: Sterile filtered

Immunogen:

Immunogen: CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Anwendungsinformationen

Applikationshinweise:

Gel Shift Dilution: 0.5 to 1.0 μLper assay

Application Note: Anti-CREB-1 Antibody has been tested in western blot and suitable in ELISA assays. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:1,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 2.56 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-CREB1 Antikörper (ABIN97864)

Chen, Garvin, Nickola, Watson, Colberg-Poley, Rose: "IL-1β induction of MUC5AC gene expression is mediated by CREB and NF-κB and repressed by dexamethasone." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 8, pp. L797-807, (2014) (PubMed).

Wierda, Kuipers, van Eggermond, Benard, van Leeuwen, Carluccio, Geutskens, Jukema, Marquez, Quax, van den Elsen: "Epigenetic control of CCR5 transcript levels in immune cells and modulation by small molecules inhibitors." in: Journal of cellular and molecular medicine, Vol. 16, Issue 8, pp. 1866-77, (2012) (PubMed).

Wang, Sasaki, Zhou, Matsumura, Araki, Mezawa, Takai, Chen, Ogata: "Transcriptional regulation of bone sialoprotein gene by interleukin-11." in: Gene, (2011) (PubMed).

Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).

Details zu CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Andere Bezeichnung: CREB1 (CREB1 Produkte)

Hintergrund: Background: Anti CREB-1 Antibody recognizes CREB (cAMP response element-binding), a cellular transcription factor. CREB binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the downstream genes. CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. Genes whose transcription is regulated by CREB include: c-fos, the neurotrophin BDNF (Brain-derived neurotrophic factor), tyrosine hydroxylase, and many neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone). CREB is closely related in structure and function to CREM (cAMP response element modulator) and ATF-1 (activating transcription factor-1) proteins. CREB proteins are expressed in many animals, including humans. CREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain.

Gen-ID: 1385

UniProt: P16220

Pathways: TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation