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CREB1 Antikörper (pSer133)

Dieses Anti-CREB1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CREB1 in WB, IHC und ELISA. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.
Rockland
Produktnummer ABIN97863
Hersteller Produkt- Nr.: 600-401-270

Kurzübersicht für CREB1 Antikörper (pSer133) (ABIN97863)

Target

Alle CREB1 Antikörper anzeigen
CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reaktivität

  • 267
  • 205
  • 166
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  • 1
Human, Maus, Ratte

Wirt

  • 242
  • 31
  • 1
  • 1
Kaninchen

Klonalität

  • 219
  • 56
Polyklonal

Konjugat

  • 144
  • 13
  • 9
  • 9
  • 8
  • 8
  • 6
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  • 5
  • 5
  • 5
  • 5
  • 5
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  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
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Dieser CREB1 Antikörper ist unkonjugiert

Applikation

  • 218
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  • 2
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  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Bindungsspezifität

    • 55
    • 27
    • 19
    • 17
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    • 9
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
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    • 1
    pSer133

    Hersteller Produkt- Nr.

    600-401-270

    Hersteller

    Rockland

    Verwendungszweck

    CREB phospho S133 Antibody

    Produktmerkmale

    Synonyms: rabbit anti-CREB pS133 Antibody, Cyclic AMP-responsive element-binding protein 1, Active transcription factor CREB antibody, cAMP responsive element binding protein 1 antibody, CREB 1 antibody, CREB-1, CREB1 antibody, MGC9284 antibody, Transactivator protein antibody

    Aufreinigung

    Anti-CREB pS133 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.

    Sterilität

    Sterile filtered

    Immunogen

    Immunogen: CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

    Immunogen Type: Conjugated Peptide

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-CREB pS133 antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity (1 % ) by ELISA against the non-phosphorylated form of the immunizing peptide.  This antibody was assayed against a variety of tissues including fibroblasts and B-cell lysates.  Bands of 46 and 43 kDa corresponding to phosphorylated CREB are observed in western blots.  Anti-CREB pS133 is suitable for the detection by immunoblot of phosphorylated human, mouse and rat CREB.  No cross-reactivity occurs with non-phosphorylated CREB.  For immunohistochemistry, formalin fixed, paraffin embedded human tissue shows moderate to strong nuclear staining in a variety of cells with minimal background staining.  Although not tested this antibody is likely functional by FACS and immunoprecipitation.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:6,000

    Other: User Optimized

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.064 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Philp, Chen, Lan, Meyer, Murphy, Knapp, Olfert, McCurdy, Marcotte, Hogan, Baar, Schenk et al.: "Sirtuin 1 (SIRT1) deacetylase activity is not required for mitochondrial biogenesis or peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) deacetylation following ..." in: The Journal of biological chemistry, Vol. 286, Issue 35, pp. 30561-70, (2011) (PubMed).

  • Target

    CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

    Andere Bezeichnung

    CREB1

    Hintergrund

    Background: The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMP-dependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II.  Alternate splicing of this gene results in two transcript variants encoding different isoforms.

    Gen-ID

    1385

    UniProt

    P16220

    Pathways

    TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation
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