PLK1 Antikörper (AA 331-508)

Produktnummer ABIN969580

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PLK1 in ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für PLK1 Antikörper (AA 331-508) (ABIN969580)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLK1 Antikörper ist unkonjugiert

Applikation: ELISA, Immunocytochemistry (ICC)

Klon: 1D1

Produktdetails anti-PLK1 Antikörper

Bindungsspezifität: AA 331-508

Verwendungszweck: PLK1 Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human PLK1 (AA: 331-508) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

ICC: 1/50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-PLK1 Antikörper (ABIN969580)

Lee, Rhee: "PLK1 phosphorylation of pericentrin initiates centrosome maturation at the onset of mitosis." in: The Journal of cell biology, Vol. 195, Issue 7, pp. 1093-101, (2011) (PubMed).

Li, Zhou, Ye, Cheng, Zhou, Lu, Xie: "Reduced miR-100 expression in cervical cancer and precursors and its carcinogenic effect through targeting PLK1 protein." in: European journal of cancer (Oxford, England : 1990), Vol. 47, Issue 14, pp. 2166-74, (2011) (PubMed).

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund: PLK1 is critical for the initiation of centrosome maturation. Polo-like kinases (PLKs) are a family of four serine/threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1, -2 and -3 are ubiquitously expressed, whereas PLK4 is restricted to a few tissues including the testes and the thymus. The mRNA and protein expression of PLK1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. Furthermore, PLK3 is a much more stable protein than PLK1, -2 or -4. PLK1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 overexpression correlates with cellular proliferation and poor prognosis. PLK2 and PLK3 are involved in checkpoint-mediated cell cycle arrest to ensure genetic stability. Loss-of-function mutations in these enzymes can lead to oncogenic transformation.

Molekulargewicht: 68 kDa

Gen-ID: 5347

UniProt: P53350

Pathways: Zellzyklus, M Phase