Sonic Hedgehog Antikörper (AA 26-161)

Produktnummer ABIN969567

Dieses Anti-Sonic Hedgehog-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Sonic Hedgehog in WB, IHC, ELISA und FACS. Geeignet für Human, Maus und Affe. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Sonic Hedgehog Antikörper (AA 26-161) (ABIN969567)

Target: Sonic Hedgehog (SHH)

Reaktivität: Human, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 5H4

Produktdetails anti-Sonic Hedgehog Antikörper

Bindungsspezifität: AA 26-161

Verwendungszweck: SHH Antibody

Aufreinigung: Purified antibody

Immunogen: Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-Sonic Hedgehog Antikörper (ABIN969567)

Kameda, Nakamura, Tanaka, Yamasaki, Kubo, Tanaka, Onishi, Katano: "Oestrogen receptor-alpha contributes to the regulation of the hedgehog signalling pathway in ERalpha-positive gastric cancer." in: British journal of cancer, Vol. 102, Issue 4, pp. 738-47, (2010) (PubMed).

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: SHH

Hintergrund: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Molekulargewicht: 49.6 kDa

Gen-ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development