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Sonic Hedgehog Antikörper (C-Term)

Name: Sonic Hedgehog Antikörper (C-Term)
Brand: antibodies-online
SKU: ABIN6391361
Price: 779.48 EUR
Availability: InStock

Verified SHH Reaktivität: Human, Maus WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated

ABIN6391361 (2µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN6391361 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.

ABIN6391361 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.

FACS

5 Abbildungen

Produktnummer ABIN6391361

Datenblatt (PDF)

Target Alle Sonic Hedgehog (SHH) Antikörper anzeigen

Sonic Hedgehog (SHH)
Bindungsspezifität
15

11

7

5

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
C-Term
Reaktivität
60

53

40

17

9

7

6

5

3

2

2

1

1

1

1

1

1

1
Human, Maus
Wirt
73

10

8

1
Ziege
Passende Sekundärantikörper
Klonalität
65

27
Polyklonal
Konjugat
52

10

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
Dieser Sonic Hedgehog Antikörper ist unkonjugiert
Applikation
72

31

25

14

13

11

9

7

7

7

5

4

3

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck

SHH

Sequenz

DSEALHPLGM AVK

Kreuzreaktivität

Hund, Human, Ratte

Aufreinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse

Verified

Isotyp

IgG

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SHH Reaktivität: Human, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

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SHH Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t
Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare

Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Minimize freezing and thawing.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Sonic Hedgehog (SHH)

Andere Bezeichnung

SHH (SHH Produkte)

Synonyme

HHG1 antikoerper, HLP3 antikoerper, HPE3 antikoerper, MCOPCB5 antikoerper, SMMCI antikoerper, TPT antikoerper, TPTPS antikoerper, 9530036O11Rik antikoerper, Dsh antikoerper, Hhg1 antikoerper, Hx antikoerper, Hxl3 antikoerper, M100081 antikoerper, fc83d08 antikoerper, shh antikoerper, syu antikoerper, vhh-1 antikoerper, vhh1 antikoerper, wu:fc83d08 antikoerper, Xhh antikoerper, hedgehog antikoerper, xshh antikoerper, SHH antikoerper, twh antikoerper, twhh antikoerper, sonic hedgehog antikoerper, sonic hedgehog a antikoerper, sonic hedgehog L homeolog antikoerper, sonic hedgehog protein A antikoerper, sonic hedgehog b antikoerper, SHH antikoerper, Shh antikoerper, shha antikoerper, shh.L antikoerper, shh antikoerper, shhb antikoerper

Hintergrund

SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

Gen-ID

6469

NCBI Accession

NP_000184

Pathways

Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development