Hexokinase 2 Antikörper

Produktnummer ABIN969196

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Hexokinase 2 in WB, IHC, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für Hexokinase 2 Antikörper (ABIN969196)

Target: Hexokinase 2 (HK2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Hexokinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Klon: 3D3

Produktdetails anti-Hexokinase 2 Antikörper

Verwendungszweck: HK2 Antibody

Aufreinigung: Ascitic fluid

Immunogen: Purified recombinant fragment of human HK2 expressed in E. Coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ELISA: 1/10000

FCM: 1/200 - 1/400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Referenzen für anti-Hexokinase 2 Antikörper (ABIN969196)

He, Bi, Zheng, Dai, Han, Liang, Ye, Zeng, Zhu, Zhong: "Real-time quantitative RT-PCR assessment of PIM-1 and hK2 mRNA expression in benign prostate hyperplasia and prostate cancer." in: Medical oncology (Northwood, London, England), Vol. 26, Issue 3, pp. 303-8, (2009) (PubMed).

Paudyal, Oriuchi, Paudyal, Higuchi, Nakajima, Endo: "Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography." in: Cancer science, Vol. 99, Issue 2, pp. 260-6, (2008) (PubMed).

Lim, Hao, Shaw, Patel, Szabó, Rual, Fisk, Li, Smolyar, Hill, Barabási, Vidal, Zoghbi: "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration." in: Cell, Vol. 125, Issue 4, pp. 801-14, (2006) (PubMed).

Details zu Hexokinase 2

Target: Hexokinase 2 (HK2)

Andere Bezeichnung: HK2

Hintergrund: The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose- 6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases .Hexokinase 2 is the predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Molekulargewicht: 102 kDa

UniProt: P52789

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt