STAT5A Antikörper (pTyr694)

Produktnummer ABIN968701

Dieses Maus Monoklonal-Antikörper erkennt spezifisch STAT5A in WB und ICS. Er zeigt eine Reaktivität gegenüber Human und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für STAT5A Antikörper (pTyr694) (ABIN968701)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STAT5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Intracellular Staining (ICS)

Klon: 47-Stat5

Produktdetails anti-STAT5A Antikörper

Bindungsspezifität: pTyr694

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Sheep Stat5 Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: For use in flow cytometry the conjugated formats of Anti-Human Stat5 (pY694) are recommended (PE-labeled, Alexa Fluor 488® and Alexa Fluor 647®).

Kommentare:

Related Products: ABIN968534

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-STAT5A Antikörper (ABIN968701)

Williams, Cheung, Park, Razani, Cohen, Muller, Di Vizio, Chopra, Pestell, Lisanti: "Loss of caveolin-1 gene expression accelerates the development of dysplastic mammary lesions in tumor-prone transgenic mice." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 1027-42, (2003) (PubMed).

Park, Lee, Frank, Razani, Nguyen, Parlow, Russell, Hulit, Pestell, Lisanti: "Caveolin-1-deficient mice show accelerated mammary gland development during pregnancy, premature lactation, and hyperactivation of the Jak-2/STAT5a signaling cascade." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3416-30, (2002) (PubMed).

Gouilleux, Pallard, Dusanter-Fourt, Wakao, Haldosen, Norstedt, Levy, Groner: "Prolactin, growth hormone, erythropoietin and granulocyte-macrophage colony stimulating factor induce MGF-Stat5 DNA binding activity." in: The EMBO journal, Vol. 14, Issue 9, pp. 2005-13, (1995) (PubMed).

Gouilleux, Wakao, Mundt, Groner: "Prolactin induces phosphorylation of Tyr694 of Stat5 (MGF), a prerequisite for DNA binding and induction of transcription." in: The EMBO journal, Vol. 13, Issue 18, pp. 4361-9, (1994) (PubMed).

Wakao, Gouilleux, Groner: "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response." in: The EMBO journal, Vol. 13, Issue 9, pp. 2182-91, (1994) (PubMed).

Details zu STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Andere Bezeichnung: Stat5

Hintergrund: The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian STATs have been identified: Stat 1-4, Stat5a, 5b, and Stat6. The SH3 domain of Stat5 shows extensive homology with the SH2 domains of Stat1 and Stat2. This homology leads to speculation that it may have a broader physiological role than originally anticipated and has led to its classification as a member of the Stat family. Stat5 has been further characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level. Further insight into the exact biological role of Stat5a and Stat5b was determined with the generation of Stat5a and Stat5b deficient mice. Stat5a was shown to be involved in lactogenesis and mammary development, while Stat5b was shown to be involved in growth hormone signaling and to play a role in liver gene expression. Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation. Stat5 (MGF) mRNA expression is highest in mammary gland tissue. Lower levels are found in ovary, thymus, spleen, kidney, lung, muscle, and the adrenal gland. The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response. There are at least three forms of PRLR, however, only the long form is able to activate the 92 kDa Stat5 protein by inducing phosphorylation at Tyr694. Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the beta-casein gene promoter. Stat5 activity is tightly regulated throughout gestation, lactation, and post-lactation. Treatment of activated Stat5 with a protein tyrosine phosphatase results in the loss of DNA binding activity. The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2. This antibody is routinely tested by Western blot analysis.

Molekulargewicht: 92 kDa

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation