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CHEK2 Antikörper (AA 31-234)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CHEK2 in WB und IF. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.
Produktnummer ABIN968596

Kurzübersicht für CHEK2 Antikörper (AA 31-234) (ABIN968596)

Target

Alle CHEK2 Antikörper anzeigen
CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität

  • 239
  • 86
  • 72
  • 16
  • 13
  • 7
  • 6
  • 5
  • 4
  • 2
  • 2
  • 1
Human, Maus, Ratte

Wirt

  • 220
  • 22
  • 1
Maus

Klonalität

  • 208
  • 35
Monoklonal

Konjugat

  • 114
  • 11
  • 9
  • 9
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 7
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
Dieser CHEK2 Antikörper ist unkonjugiert

Applikation

  • 204
  • 79
  • 78
  • 71
  • 44
  • 39
  • 34
  • 28
  • 24
  • 21
  • 18
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF)

Klon

19-Chk2
  • Bindungsspezifität

    • 26
    • 25
    • 19
    • 17
    • 16
    • 16
    • 15
    • 15
    • 9
    • 8
    • 8
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-234

    Kreuzreaktivität

    Human, Ratte (Rattus)

    Produktmerkmale

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

    Aufreinigung

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    Mouse Chk2 aa. 31-234

    Isotyp

    IgG1
  • Kommentare

    Related Products: ABIN967389

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    250 μg/mL

    Buffer

    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store undiluted at -20° C.
  • Tominaga, Morisaki, Kaneko, Fujimoto, Tanaka, Ohtsubo, Hirai, Okayama, Ikeda, Nakanishi: "Role of human Cds1 (Chk2) kinase in DNA damage checkpoint and its regulation by p53." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31463-7, (1999) (PubMed).

  • Target

    CHEK2 (Checkpoint Kinase 2 (CHEK2))

    Andere Bezeichnung

    Chk2

    Hintergrund

    The cell cycle is regulated by multiple checkpoints that determine cell fate. Such checkpoints ensure that DNA replication and chromosomal segregation are completed with high fidelity. When DNA is damaged, specific kinases and phosphatases, key components of cell cycle checkpoints, function to arrest the cell cycle and provide the necessary time for DNA repair. For example, DNA damage induces arrest of the cell cycle at the G2 checkpoint via Wee1-mediated inhibitory phosphorylation of the kinase Cdc2. Inhibition is relieved by the Cdc25C phosphatase via Cdc2 dephosphorylation. In turn, the Chk1 and Chk2 protein kinases phosphorylate and inhibit Cdc25C, thus preventing activation of the Cdc2-cyclin B complex and entry into mitosis. Chk2, the human homolog of S. cerevisiae Rad53 and S. pombe Cds1, contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA) which is found in other cell cycle kinases. Chk2 is expressed during late G1 to M phase and is found in the nucleoplasm. Thus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage. This antibody is routinely tested by western blot analysis.

    Molekulargewicht

    60 kDa

    Pathways

    p53 Signalweg, Apoptose, Zellzyklus
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