RAC1 Antikörper

Produktnummer ABIN968089

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RAC1 in WB, IHC, IF und IP. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund und Huhn und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für RAC1 Antikörper (ABIN968089)

Target: RAC1

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 102-Rac1

Produktdetails anti-RAC1 Antikörper

Kreuzreaktivität: Ratte (Rattus), Huhn, Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rac1 Protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968545

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-RAC1 Antikörper (ABIN968089)

Habas, Dawid, He: "Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation." in: Genes & development, Vol. 17, Issue 2, pp. 295-309, (2003) (PubMed).

Doye, Mettouchi, Bossis, Clément, Buisson-Touati, Flatau, Gagnoux, Piechaczyk, Boquet, Lemichez: "CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion." in: Cell, Vol. 111, Issue 4, pp. 553-64, (2002) (PubMed).

Innocenti, Tenca, Frittoli, Faretta, Tocchetti, Di Fiore, Scita: "Mechanisms through which Sos-1 coordinates the activation of Ras and Rac." in: The Journal of cell biology, Vol. 156, Issue 1, pp. 125-36, (2002) (PubMed).

Nobes, Hall: "Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia." in: Cell, Vol. 81, Issue 1, pp. 53-62, (1995) (PubMed).

Didsbury, Weber, Bokoch, Evans, Snyderman: "rac, a novel ras-related family of proteins that are botulinum toxin substrates." in: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16378-82, (1989) (PubMed).

Details zu RAC1

Target: RAC1

Hintergrund: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, like many of its relatives, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. In addition, IQGAP1 has been identified as a GTPase activating protein specific for Rac1. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 21 kDa

Pathways: WNT Signalweg, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling