RAC1 Antikörper

Produktnummer ABIN968088

Der Maus Monoklonal anti-RAC1 Antikörper (Klon 102-Rac1) (ABIN968088) detektiert spezifisch RAC1 in WB, IHC, IF und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Hund und Huhn.

Kurzübersicht für RAC1 Antikörper (ABIN968088)

Target: RAC1

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 102-Rac1

Produktdetails anti-RAC1 Antikörper

Kreuzreaktivität: Huhn, Hund, Maus, Ratte (Rattus)

Produktmerkmale: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Positive Control: Rat Cerebrum

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, glycerol.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Details zu RAC1

Target: RAC1

Hintergrund: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. IQGAP1 has been identified as a GTPase activating protein specific for Rac1.
Testing in native ELISA with recombinant proteins to CDC42 and Rho have shown that the antibody to Rac1 (cat No ABIN968088/1) recognizes CDC42 and Rho. Due to sequence homology among various small GTPase familyl members, potential reactivity could be observed with other family members.

Molekulargewicht: 21 kDa

Pathways: WNT Signalweg, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling