ENOS Antikörper (AA 1025-1203)

Produktnummer ABIN967886

Der Maus Monoklonal Anti-ENOS-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, ENOS in Proben von Human, Ratte und Maus zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für ENOS Antikörper (AA 1025-1203) (ABIN967886)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ENOS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 3-eNOS-NOS Type III

Produktdetails anti-ENOS Antikörper

Bindungsspezifität: AA 1025-1203

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human eNOS aa. 1025-1203

Isotyp: IgG1 kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN968536, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ENOS Antikörper (ABIN967886)

Kincer, Uittenbogaard, Dressman, Guerin, Febbraio, Guo, Smart: "Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23525-33, (2002) (PubMed).

Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, (2002) (PubMed).

Cao, Yao, McCabe, Yao, Katusic, Sessa, Shah: "Direct interaction between endothelial nitric-oxide synthase and dynamin-2. Implications for nitric-oxide synthase function." in: The Journal of biological chemistry, Vol. 276, Issue 17, pp. 14249-56, (2001) (PubMed).

Laufs, Endres, Stagliano, Amin-Hanjani, Chui, Yang, Simoncini, Yamada, Rabkin, Allen, Huang, Böhm, Schoen, Moskowitz, Liao: "Neuroprotection mediated by changes in the endothelial actin cytoskeleton." in: The Journal of clinical investigation, Vol. 106, Issue 1, pp. 15-24, (2000) (PubMed).

Zhao, Dugas, Mathiot, Delmer, Dugas, Sigaux, Kolb: "B-cell chronic lymphocytic leukemia cells express a functional inducible nitric oxide synthase displaying anti-apoptotic activity." in: Blood, Vol. 92, Issue 3, pp. 1031-43, (1998) (PubMed).

Details zu ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Andere Bezeichnung: eNOS/NOS Type III

Hintergrund: Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca[2+] levels and enhance calmodulin binding. Neuronal NOS (mNOS) and endothelial NOS (eNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and both are regulated in a similar manner. The human forms exhibit 52% amino acid identity. However, they are distinct gene products of about 155 kDa (mNOS) and 140 kDA (eNOS). The eNOS gene was cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC). eNOS protein has a unique N-myristylation consensus sequence that may explain its membrane localization.

Molekulargewicht: 140 kDa

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling