p130 Antikörper (AA 26-367)

Produktnummer ABIN967863

Dieses Anti-p130-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von p130 in WB, IF, IHC und IP. Geeignet für Human, Ratte, Maus, Blow Fly und Huhn. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für p130 Antikörper (AA 26-367) (ABIN967863)

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reaktivität: Human, Ratte, Maus, Blow Fly, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser p130 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Klon: 10-Rb2

Produktdetails anti-p130 Antikörper

Bindungsspezifität: AA 26-367

Kreuzreaktivität: Maus, Ratte (Rattus), Huhn, Fruchtfliege (Drosophila melanogaster)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rb2 aa. 26-367

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-p130 Antikörper (ABIN967863)

Fusaro, Wang, Chellappan: "Differential regulation of Rb family proteins and prohibitin during camptothecin-induced apoptosis." in: Oncogene, Vol. 21, Issue 29, pp. 4539-48, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Laplantine, Rossi, Sahni, Basilico, Cobrinik: "FGF signaling targets the pRb-related p107 and p130 proteins to induce chondrocyte growth arrest." in: The Journal of cell biology, Vol. 158, Issue 4, pp. 741-50, (2002) (PubMed).

Yeung, Bell, Testa, Mayol, Baldi, Graña, Klinga-Levan, Knudson, Giordano: "The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19." in: Oncogene, Vol. 8, Issue 12, pp. 3465-8, (1993) (PubMed).

Details zu p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Andere Bezeichnung: Rb2

Hintergrund: The pRb2 protein shows a high degree of identity with pRb and the related p107 protein. Both pRb and p107 bind to the adenovirus E1A, SV40 large T antigen, and papillomavirus E7 viral proteins. This binding initiates the release of transcription factors which are required for the expression of cell cycle-regulated genes. pRb, pRb2, and p107 interact with a conserved motif in these three viral proteins. In the E1A protein, this area is known as transforming domain 2, which is required for growth activation. The E1A-binding domain in pRb and p107 is a conserved motif known as the pocket region which consists of conserved A and B regions separated by non-conserved spacers of different sizes in pRb and p107. It is the pocket regions of pRb and p107 that associate with the E2F transcription factor. pRb2 also contains the conserved pocket region suggesting that it has functional similarities to pRb and p107. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 130 kDa

Pathways: Zellzyklus, Mitotic G1-G1/S Phases